Lipid composition of sunflower seeds (Helianthus annuus L.) using multivariate analysis
Titel:
Lipid composition of sunflower seeds (Helianthus annuus L.) using multivariate analysis
Auteur:
Auristela MALAVÉ ACUÑA Jesús Rafael MÉNDEZ NATERA
Verschenen in:
Revista científica UDO agrícola
Paginering:
Jaargang 6 (2006) nr. 1 pagina's 27-32
Jaar:
2006
Inhoud:
The objective of this work was to compare by multivariate techniques three experimental cultivars of sunflower (B-5, B-9 yB-16). Seed lipids were extracted with a chloroform-methanol mixture (2:1 v/v). For their subsequent analyses byautomated thin layer chromatography, with flame ionization detector (TLC/FID), and gas-liquid chromatography tocharacterize and quantify the total lipids and fatty acids, respectively. Percentage of total lipids, lipid composition, viz,triacylglycerol, diacylglycerol, phospholipids and fatty acids composition, viz, palmitic, araquídic, oleic, linoleic, linolenicand eicosenoic acids were determined. Principal component analyses and cluster analyses were carried out. For the principalcomponent, the first component explained 79.52 % of the variation and the second one explained 20.41 % (total 100,00 %),cultivars B-5 y B-16 were more related to each other, while the most important characters were phospolipids andtriacylglycerol indicating a bigger variability among cultivars for these characters, while the less important ones werelinolenic acid (C18:3) and eicosenoic acid (C20:1). Cluster analysis indicated similar results to those of principalcomponents. Principal component and cluster analysis should be used to study the relationships among total lipids, lipidcomposition and fatty acids in order to identifying similar groups for these characters for different sunflower cultivars.
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