Disinfection of cassava (Manihot esculenta Crantz) shoots cv. ‘Querepa Rosada’ with sodium hypochlorite
Title:
Disinfection of cassava (Manihot esculenta Crantz) shoots cv. ‘Querepa Rosada’ with sodium hypochlorite
Author:
José Alberto LAYNEZ GARSABALL María Claudia SÁNCHEZ CUEVAS
Appeared in:
Revista científica UDO agrícola
Paging:
Volume 6 (2006) nr. 1 pages 60-66
Year:
2006
Contents:
This research was conducted in order to develop a disinfection protocol for apical shoots of cassava, cultivar ¨QuerepaRosada¨, using three dilutions of sodium hypochlorite and three immersion periods. Meristems, 1mm long, from apicalshoots collected in a commercial cassava plantation located in Jusepin, Monagas State, were used. The statistical designwas completely randomized, with 11 treatments, 10 repetitions, one explant per repetition and for test tube. Treatmentsused were three sodium hypochlorite concentrations (a.i. 5.25%): 10, 20, and 30% (C10, C20 Y C30, respectively),combined with three immersion times: 10, 20 y 30min (T10, T20 and T30, respectively). Two control treatments wereincluded: ethanol 70% for 5 sec and mercury chloride for 10min. Murashige and Skoog (1962) culture medium was used,suplemented with sucrose (20 g/L), thiamine-HCl (0,4 mg/L), m-Inositol (100 mg/L), BAP (0,05 mg/L), AG (0,05 mg/L),ANA (0,02 mg/L), agar (7 g/L), and a 5.8 pH. Explants were maintained in a growth chamber at a 27-30ºC and 16hr ofartificial light. The percent of alive and not contaminated shoots was evaluated 8, 16 and 24 days after planting. Treatmentdifferences were established using t-student at 0.05%. The best disinfection treatment, 24 days after planting, was the 10%(v/v) solution of sodium hypochlorite during 10 min.
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